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Expression of proliferating cell nuclear antigen in mouse skin at different time points following ultraviolet B exposure SR Palep, RN Saladi, J Moore, M Lebwohl and H Wei Dermatology, Mount Sinai School of Medicine, New York, NY Proliferating cell nuclear antigen PCNA ; is involved in DNA replication, recombination, and repair. PCNA is found throughout the basal layer in normal skin, however in malignant skin lesions PCNA has been seen in all layers of the epidermis and dermis. We previously reported that PUVA and UVB exposure diminished the expression of PCNA in mouse skin. The aim of this study is to evaluate PCNA expression at different time points following exposure to both acute and chronic levels of UVB. In part I of this study SKH-1 mice 3 group ; were sacrificed at the following time points: 6, 12, 24, hrs, and 7 days after exposure to UVB 1.5kj m2. In Part II of this study mice were sacrificed at the time points: 6, 12, and 24 hrs after chronic UVB exposure of 3kj m2. Immunohistochemistry was performed on paraffin skin sections with anti-PCNA antibody. PCNA expression was quantitated by measuring the number of positively stained cells per 100 keratinocytes. In Part I 1.5kJ m2 UVB ; , the results showed the usual pattern of PCNA throughout the basal layer of normal skin. Significantly diminished expression was seen in 6hrs, subsequent time points, 12 hrs to 7 days, PCNA positive cells were progressively increased with pagetoid distribution in all layers of the epidermis. The diminished PCNA expression at the 6 hrs time point is possibly due to the skin undergoing immediate damage in response to UVB. Progressive reactivation of PCNA expression is a result of repair mechanisms initiated by the immune system. In Part II 3kJ m2 UVB ; , at 6, 12, and 24 hrs, although there was a progressive increase of PCNA positive cells, the relative number of positive cells were decreased in comparison to Part I. The decrease in the number of PCNA positive stained cells is in response to the chronic levels of UVB. The progressive pagetoid extension of the PCNA cells may indicate a malignant transformation. In conclusion, PCNA can serve as a marker associated with DNA damage and repair, the expression being dose dependent and time related, for example, topamax long term side effects.
This study has been approved by the medical research ethics committee of the University of Queensland in accordance with the National Health and Medical Research Council's guidelines. You are of course, free to discuss your participation in this study with project staff contactable on 1300 889 618 ; . If you would like to speak to an officer of the University not involved in the study, you may contact the Ethics Officer on 07 3365 3924.
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The NHLBI's Nonhuman Primate Models Program at the Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina, announces the availability of plasma samples from nonhuman primates with disorders in the pathogenesis of atherosclerosis. The models are available for lipoprotein and other studies: Cynomolgus macaques macaca fascicularis ; fed diets containing 0.4 mg cholesterol per kcal whose serum cholesterol response are hyper- about 400 mg dl ; or hypo- 200 mg dl ; . Cynomolgus macaques fed a moderately atherogenic or control diet that are either obese or lean. Obese or lean rhesus monkeys macaca mulatta ; consuming an atherogenic diet. Insulin-dependent Type I diabetic cynomolgus macaques fed either a moderately atherogenic or control diet. Cynomolgus monkeys CyLA identical or haplo-identical fed a control or a moderately atherogenic diet. Postmyocardial infarction cases of rhesus monkeys consuming a moderately atherogenic diet. Young rhesus monkeys with minimal or moderate coronary artery atherosclerosis with comparable plasma lipid concentrations.
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325-329 14. Durieux, M.E., Carlisle, S.J., Salafranca, M.N., and Lynch, K.R. 1993 ; Am. J. Physiol. 264, C1360-C1364 15. Hooks, S.B., Ragan, S.P., Hopper, D.W., Honemann, C.W., Durieux, M.E., MacDonald, T.L., and Lynch, K.R. 1998 ; Mol. Pharmacol. 53, 188-194 16. Ungrin, M.D., Singh, L.M., Stocco, R., Sas, D.E., and Abramovitz, M. 1999 ; Anal. Biochem. 272, 34-42 17. Frey, E.A., Nicholson, D.W., Metters, and K.M. 1993 ; Eur. J. Pharmacol. 244, 239250 18. Marchese, A., Docherty, J.M., Nguyen, T., Heiber, M., Cheng, R., Heng, H.H., Tsui, L.C., Shi, X., George, S.R., and O'Dowd, B.F. 1994 ; Genomics 23, 609-18 19. Chomczynski, P. and Sacchi, N. 1987 ; Anal. Biochem. 162, 156-9 20. Yokomizo, T., Izumi, T., Chang, K., Takuwa, Y., Shimizu, T. 1997 ; Nature 387, 620-624 and zantac.
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Single copies of this report will be available free until November 22, 1996, from the CDC National AIDS Clearinghouse, P Box 6003, Rockville, MD 20849-6003; telephone 800 ; 458.O. 5231 or 301 ; 217-0023. Northeast Connecticut, Maine, Massachusetts, New Hampshire, New Jersey, New York, Pennsylvania, Rhode Island, and Vermont; Midwest Illinois, Indiana, Iowa, Kansas, Michigan, Minnesota, Missouri, Nebraska, North Dakota, Ohio, South Dakota, and Wisconsin; South Alabama, Arkansas, Delaware, District of Columbia, Florida, Georgia, Kentucky, Louisiana, Maryland, Mississippi, North Carolina, Oklahoma, South Carolina, Tennessee, Texas, Virginia, and West Virginia; West Alaska, Arizona, California, Colorado, Hawaii, Idaho, Montana, Nevada, New Mexico, Oregon, Utah, Washington, and Wyoming. U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES Public Health Service and ceclor.
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Dear Nagarathars: Greetings, I want to welcome all of you to our national retreat in May at Downers Grove, IL Chicago area ; , this will be a great opportunity for all of us to come together. Again NSNA and its members have proved it to the society that they will respond to a call for help. In January, our team acted swiftly to establish a Disaster Relief effort to respond to the calamities of the world "Tsunami". We have been supporting the relief activities by contributing to the cause, this is all made possible because of you the members ; and I want to congratulate you on the accomplishment. This is a feat accomplished with the support and willingness provided by many of our Nagarathars. Our Tsunami relief effort started with a contribution of $10 K made by Mr. & Mrs. Arun Alagappan, followed by several Nagarathars who have contributed substantially to the cause and have been behind NSNA to lend the helping hand. Our activities have been around funding the Hindu Relief Fund, working with the Thiruvetriyur Nagarathar Sangam in the rebuilding efforts of local native residences and supporting several affected Nagarathar families. Membership: I would like to bring to your attention and earnest cooperation regarding membership. Please upgrade your membership to be a "Life Member" if you are not. If you are a life member, we would like to thank you for your support. There are plans to publish national membership directory with addresses of members. I would like to solicit the interest level regarding this, please email me at Secretary achi if you are not interested in being listed. I want to thank each and every one of you for making NSNA a successful institution. "You can accomplish anything in life, provided that you do not mind who gets the credit" Karthik Swarnam.
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